Staff directory

Javier García Pardo

SO Postdoctoral Researcher
javier.garcia(ELIMINAR)@icn2.cat
Nanostructured Functional Materials

Publications

2017

  • Conserved effects and altered trafficking of Cetuximab antibodies conjugated to gold nanoparticles with precise control of their number and orientation

    García-Fernández L., Garcia-Pardo J., Tort O., Prior I., Brust M., Casals E., Lorenzo J., Puntes V.F. Nanoscale; 9 (18): 6111 - 6121. 2017. 10.1039/c7nr00947j. IF: 7.367

    Gold nanoparticles (17 nm) have been functionalized with the antiangiogenic monoclonal antibody drug Cetuximab at a well-defined orientation and coverage density of antibodies. Functionalization has been carried out through site-directed chemistry via the selective oxidation of the carbohydrate moiety of antibodies linked to a thiolated hydrazide. A431 tumor cells have been exposed to these conjugates for in vitro evaluation of their effects. In addition to epithelial growth factor receptor blocking, trafficking and signaling alterations were also observed. Thus, the blocking effects of Cetuximab were increased and sustained for a longer time when associated with the nanoparticles. Enhancing antibody therapy effects by decreasing the needed dose and prolonging its effect by avoiding receptor recycling may serve to obtain increased therapeutic benefits for immunotherapy. © The Royal Society of Chemistry 2017.


2016

  • Biocompatible polydopamine-like particles for the removal of heavy metals at extremely low concentrations

    Contreras Rodríguez A.R., Saiz-Poseu J., García-Pardo J., García B., Lorenzo J., Ojea-Jiménez I., Komilis D., Sedó J., Busqué F., Sánchez A., Ruiz-Molina D., Font X. RSC Advances; 6 (46): 40058 - 40066. 2016. 10.1039/c6ra03664c. IF: 3.289

    A family of catechol-based submicron particles, with sizes between 200 and 300 nm, was tested for the removal of Cd(ii), Pb(ii) and Cr(vi) in water. The highest adsorption capacity was obtained with catechol-based particles in the case of Pb(ii), followed by Cd(ii). However, the catechol particles failed to adsorb Cr(vi). Our results indicate an up to four-fold increase of the adsorption capacity of these particles compared to that of activated carbon under the same experimental conditions. To check the biocompatible character of the submicron particles, their stability was evaluated in a phosphate buffer solution (PBS) and in a cell culture medium. The results confirmed that the presence of proteins in the medium favors their stability. A bioluminescent Vibrio fischeri test and a cytotoxicity assay on the HepG2 cell line were used to determine that the catechol particles did not exhibit any substantial toxicity. The results show that these catechol-based particles can be used as an efficient biocompatible adsorbent to remove heavy metals at extremely low concentrations. © The Royal Society of Chemistry 2016.