Title
Microscopy sample preparation Lab
Technique
Optical Microscopy
Description
For achieving an excellent TEM sample we need of nearly zero thickness in the observable zone of our sample. This is done making an upper and down concavity using ion milling. This process ends in a small hole with shores with a thickness under the 20nm, therefore transparent to electrons. Is capital to stop the milling before the hole grows, increases the thickness gradient and ruins the electron transparency of the sample. To now the perfect moment to stop we use the birefringence properties of our materials. In the image we can see a perfect Michel-Levy Chart where the interference of the light offers an espectacle that showns how are related the cascade colours and the thickness of the concavity.

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Technical specifications
For making this image we have use a Leica DM 2700M optical microscope situated in the sample preparation laboratory.
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