24 July

Development and application of innovative point-of-care biosensing platforms

Friday 24 July 2020, 11:00am

ICN2 Seminar Hall, ICN2 Building, UAB


PhD: Amadeo Sena

Directors: Prof. Arben Merkoçi, Group Leader and Dr Ruslan Alvarez, Postdoctoral Researcher at Nanobioelctronics and Biosensors Group at ICN2.

Short Abstract: This thesis is devoted to the development of innovative paper-based nanobiosensors for application at the Point-of-Care (PoC). Particularly, it focuses on one of the most successful PoC tests: the lateral flow assay (LFA). Key aspects of the assay, such as components and reagents, manufacturing and operating procedures are covered in depth. In addition, the thesis provides a comprehensive review of the challenges that LFA has faced and the most relevant improvements reported in recent years.

Three biosensing platforms have been developed for application at the PoC. A paper-based electrophoretic bioassay has been designed combining the key features of a lateral flow assay with the capabilities of electrophoresis sample treatment. The biosensor has been developed and applied for the detection of H-IgG in whole blood samples. The second work is a simple and low-cost strategy designed for the improvement of the analytical sensitivity of LFA. It is based on the integration of a dissolvable wax barrier on the strip enabling an extended internal incubation step to promote the immunocomplex formation in the test line. Finally, an innovative lab in a tube fluorescent biosensor comprising a microtube with a nano-conjugated fiberglass has been developed for the detection of Escherichia coli in river and tap water. The detection principle is based on Förster resonance energy transfer using gold nanoclusters as a signal reporter and antibody-conjugated gold nanoparticles as a quencher. This work has demonstrated high selectivity and the lowest limits of detection reported so far in real samples using a PoC biosensor.