18 January

Designing advanced nanomaterials for next generation in vitro diagnostics: development of optical and electrochemical biosensors based on hollow AuAg nanoparticles for determination of viral and bacterial infections

Friday 18 January 2019, 11:00am

ICN2 Seminar Hall, ICN2 Building, UAB

PhD: Lorenzo Russo

Directors: ICREA Prof. Arben Merkoçi, Nanobioelectronics and Biosensors Group Leader and ICREA Prof. Victor F. Puntes, Inorganic Nanoparticles Group Leader at ICN2

Short Abstract: In this PhD thesis, the rational design of advanced nanomaterials with controlled properties was applied for their employment in biosensing, leading to the development of two diagnostic platforms for the determination of viral and bacterial infections. A highly reproducible and robust synthetic method for the production of monodisperse AuAg alloy nanoshells based on galvanic replacement reaction was developed. The protocol described allows the precise control over the particles’ morphology, in terms of shell thicknesses and void sizes, the relative composition and topological distribution of their constituting noble metals, as well as their surface roughness and porosity.

Taking advantage of the tunable plasmonic properties of AuAg NSs, the development of a SERS-based lateral flow assay for the sensitive and quantitative detection of MxA, a biomarker commonly associated to viral infections, was achieved down to the analytically-relevant LOD of few ng/mL.

Moreover, the capability to precisely modulate AuAg NSs elemental composition lead to the design of a proof-of-concept electrochemical assay for the rapid detection of two model bacterial strains, Escherichia coli and Salmonella typhimurium.