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Monday, 27 April 2015

ICN2 collaborates in a study about the interaction of carbon nanotubes and the blood-brain barrier

ICN2 Electron Microscopy Division, led by Dr Belén Ballesteros, has participated in a research which studies the interaction of carbon nanotubes with an in vitro blood-brain barrier model and a mouse brain in vivo. The results of the study have been published in Biomaterials.

A research published in Biomaterials studies the interaction of carbon nanotubes and the blood-brain barrier through two different proceedings. The study was carried by the Institute of Pharmaceutical Science at the King's College London and Elzbieta Pach and Belén Ballesteros, members of the ICN2 Electron Microscopy Division, participated on the electron microscopy characterization studies.

The study investigates the ability of amino-functionalized multi-walled carbon nanotubes (MWNTs-NH3+) to cross the Blood-Brain Barrier (BBB) by two ways: in vitro using a co-culture BBB model comprising primary porcine brain endothelial cells (PBEC) and primary rat astrocytes and, in vivo, following a systemic administration of radiolabelled f-MWNTs.

The study carried out at ICN2 has allowed the corroboration of the results and the better understanding of the processes. Images by Transmission Electron microscopy (TEM) and Scanning Transmission Electron Microscopy (STEM) showed that the cells or the tight junction assemblies were not damaged, that the interaction of MWNTs-NH3+ and the plasma membrane of the endothelial cells took place after 4 h of incubation and confirmed that MWNTs-NH3+ crossed the PBEC monolayer via energy-dependent transcytosis. Also, high resolution TEM (HRTEM) and Electron Energy Loss Spectroscopy (EELS) showed that the graphitic structure of the MWNTs-NH3+ was preserved following uptake into PBEC.

To sum up, researchers were able to demonstrate, for the first time, the ability of MWNTs-NH3+ to cross the BBB in vitro with low voltage STEM imaging, thus providing solid evidence using electron microscopy for each step of the transcytosis process. This research also stands out because its results could lead to the use of CNTs in new applications. For instance, they could work as nanocarriers for delivery of drugs and biologics to the brain, after systemic administration.

 

Article Reference

Houmam Kafaa, Julie Tzu-Wen Wanga, Noelia Rubioa, Kerrie Vennerb, Glenn Andersonc, Elzbieta Pachd, Belén Ballesterosd, Jane E. Prestona, N. Joan Abbotta, Khuloud T. Al-Jamala. (2015), The interaction of carbon nanotubes with an in vitro blood-brain barrier model and mouse brain in vivo. Biomaterials, 53, p 437–452. doi:10.1016/j.biomaterials.2015.02.083